Document 0085
 DOCN  M9490085
 TI    Targeted lymph node immunization with simian immunodeficiency virus p27
       antigen to elicit genital, rectal, and urinary immune responses in
       nonhuman primates.
 DT    9411
 AU    Lehner T; Bergmeier LA; Tao L; Panagiotidi C; Klavinskis LS; Hussain L;
       Ward RG; Meyers N; Adams SE; Gearing AJ; et al; Department of
       Immunology, United Medical School, Guy's Hospital,; London, United
       Kingdom.
 SO    J Immunol. 1994 Aug 15;153(4):1858-68. Unique Identifier : AIDSLINE
       MED/94321795
 AB    A s.c. route of immunization was developed in non-human primates, which
       targets the genitourinary-rectal associated lymphoid tissue. A vaccine
       consisting of rSIV gag p27, expressed as hybrid Ty virus-like particles
       (p27: Ty-VLP) was administered in the proximity of the internal iliac
       lymph nodes. Secretory IgA and IgG Abs to the p27 Ag were elicited in
       the vaginal, male urethral, rectal and seminal fluids, urine and serum.
       Two or more immunodominant B cell epitopes were identified within
       peptides 51-90 and 121-170 of the sequence of p27, using serum or
       biliary IgA and IgG Abs. CD4+ T cell proliferative responses to p27 were
       elicited predominantly in the targeted internal iliac, as well as the
       inferior mesenteric lymph nodes and the spleen, but not in the unrelated
       lymph nodes. These cells were then studied for helper function in p27
       specific B cell Ab synthesis. Specific IgA and IgG Abs were detected in
       the same lymphoid tissues as those that displayed proliferative
       responses. However, cross-over reconstitution experiments between
       splenic and iliac lymph node B and CD4+ T cells suggest that the iliac B
       cells are essential for specific IgA Ab synthesis, whereas splenic B
       cells preferentially synthesize IgG Ab. The targeted lymph node (TLN)
       route of immunization gave comparable B cell, proliferative T cell, and
       Th cell responses to the vaginal, male genitourinary, and rectal mucosal
       routes, which were augmented by oral immunization. However, the TLN
       route induced urinary and seminal fluid sIgA and IgG Abs in addition to
       genital and rectal Abs. Generating secretory IgA and IgG Abs at the
       mucosal surfaces, and T and B cell immunity in the regional draining
       lymph nodes, spleen and circulation by TLN immunization may prevent
       transmission of virus through the mucosa, dissemination of the virus,
       and the formation of a latent reservoir of infection.
 DE    Animal  Antibodies, Viral/*IMMUNOLOGY  Antigenic Determinants  Antigens,
       Viral/*IMMUNOLOGY  Binding, Competitive  Female  Gene Products,
       gag/*IMMUNOLOGY  IgA/IMMUNOLOGY  IgG/IMMUNOLOGY  Lymph Nodes/*IMMUNOLOGY
       Lymphocyte Transformation  Lymphoid Tissue/*IMMUNOLOGY  Macaca mulatta
       Male  Rectum/*IMMUNOLOGY  Spleen/IMMUNOLOGY  Support, Non-U.S. Gov't
       SIV/*IMMUNOLOGY  T-Lymphocytes, Helper-Inducer/IMMUNOLOGY  T4
       Lymphocytes/IMMUNOLOGY  Urogenital System/*IMMUNOLOGY  Vaccines,
       Synthetic/ADMINISTRATION & DOSAGE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).